Addgene is a US-based nonprofit plasmid repository. Addgene’s mission is to accelerate research and discovery by improving access to useful research materials and information. Addgene facilitates the sharing of high-quality scientific materials, research reproducibility, and open science by archiving and distributing DNA-based research reagents and associated data to scientists worldwide. Addgene’s repository includes special collections on CRISPR, fluorescent proteins, viral plasmids, and more. Addgene also provides ready-to-use AAV and lentiviral preparations of commonly requested plasmids. Addgene offers free online educational resources and protocols about molecular biology such as vector backbones, cloning, CRISPR, and AAV and lentivirus production.