With the development of advanced imaging techniques, the number of biological images acquired in digital form is growing rapidly, resulting in large-scale bioimage databases. The systematic and high-throughput analysis and mining of the information in such bioimage databases is vital to the success of a wide spectrum of studies in molecular biology, from intracellular processes to whole-organ cell-resolution models. The goals of this conference are (1) to bring together interdisciplinary researchers to present the latest advances in image data analysis and informatics methods that are, or might be, relevant to such bioimage analysis problems, and (2) to provide a forum for an in-depth discussion of the challenges in this area and their possible solutions.